How to avoid organisms with lipids from floating in the scanning tray

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How to avoid organisms with lipids from floating in the scanning tray

Post  Carmen Garcia-Comas on Thu Dec 16, 2010 11:07 am

Hi,

Today a colleague asked me about how preventing organisms from floating (i.e., copepods with lipid reserves).
I have always had a few organisms floating and I just pushed them one by one with my cactus needle and that works well.
But what to do when you have your sample packed with hundreds of copepods floating in the surface?!

I remember once I saw a researcher putting a very little amount of soap (your needle covered with a thin layer of liquid soap) in the tray once the sample was there. Once diluted in the water, the organisms were easier to sink. But I would like to have any alternative tips.

How do you do it in your lab?

Cheers

Carmen

Carmen Garcia-Comas

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